MAJIQ: Modeling Alternative Junction Inclusion Quantification
MAJIQ and Voila are two software packages that together detect, quantify, and visualize
local splicing variations (LSV) from RNA-Seq data. Conceptually, MAJIQ/Voila can be divided into
- MAJIQ Builder: Uses RNA-Seq (BAM files) and a transcriptome annotation file (GFF3) to
define splice graphs and known/novel Local Splice Variations (LSV).
- MAJIQ Quantifier: Quantifies relative abundance (PSI) of LSVs and changes in relative LSV abundance
(delta PSI) between conditions w/wo replicates.
Voila: A visualization package that combines the output of MAJIQ Builder and MAJIQ Quantifier using
interactive D3 components and HTML5. Voila creates interactive summary files with gene splice graphs,
LSVs, and their quantification.
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